Instructions

Monitor Drug Conc. vs Time

Create a Fluorescein Standard Curve (Indirect Measurements) This instruction manual outlines the use of SciFlow 1000 and including fluorescein as a tracer dye to extrapolate concentrations of compounds (concentration vs. time) across linked cell compartments as fluid moves across a channel. Once a standard curve is generated, a line equation is calculated. This equation allows back calculation of an absolute fluorescein concentration which is then correlated to the starting concentration of a drug or toxicant. To access Click Here